Regulation of the L-arabinose transport operons in Escherichia coli.

Using L-arabinose for Production of Human Growth Hormone in Escherichia coli, Studying the Processing of gIII::hGH Precursor

AraC protein, regulation of the l-arabinose operon in Escherichia coli, and the light switch mechanism of AraC action.

This review covers the physiological aspects of regulation of the arabinose operon in Escherichia coli and the physical and regulatory properties of the operon's controlling gene, araC. It also describes the light switch mechanism as an explanation for many of the protein's properties. Although many thousands of homologs of AraC exist and regulate many diverse operons in response to many differ...

L-arabinose transport systems in Escherichia coli K-12.

Mutations in the arabinose transport operons of Escherichia coli K-12 were isolated with the Mu lac phage by screening for cells in which beta-galactosidase is induced in the presence of L-arabinose. Standard genetic techniques were then used to isolate numerous mutations in either of the two transport systems. Complementation tests revealed only one gene, araE, in the low-affinity arabinose up...

Nonsense mutants in the regulator gene araC of the L-arabinose system of Escherichia coli B-r.

HE L-arabinose-gene complex in Escherichia coli B/r is a regulon (MAAS and CLARK 1964) corisisting of at least two operons controlled by regulator gene araC. One operon consists of structural genes, araD (L-ribulose 5-phosphate 4-epimerase), araA (L-arabinose isomerase), and araB (L-ribulokinase) , and controlling sites, araZ (initiator) and araO (operator). These genetic units are linked in th...

Metabolite gene regulation of the L-arabinose operon in Escherichia coli with indoleacetic acid and other indole derivatives.

The ability of indole derivatives to facilitate RNA polymerase transcription of the L-arabinose operon in Escherichia coli was shown to require the catabolite activator protein (CAP) as well as the araC gene product. Adenosine 3',5'-monophosphate (cAMP) was not obligatory for araBAD transcription when the cells were grown in the presence of 1 mM indole-3-acetic acid or in the presence of indole...